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Spontaneously Immortalized Avian Cell Line

High affinity monoclonal antibodies against Shiga toxin strain Stx2 and hybridomas that produce such antibodies are described. The antibodies may be used in a kit for detecting Stx2 and variants thereof in a sample.

• The hybridoma cell lines produce monoclonal antibodies that detect all four variants of Stx2
Immunoassays are rapid, highly specific and sensitive

• Basis for developing a sensitive immunoassays for detecting variants for Shiga toxin 2
• Immunoassys could be used for monitoring and source-tracking food supplies as well as monitoring contamination of clinical and environmental samples such as feces, soil, air, and water.

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