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A SYSTEM FOR HIGH THROUGHPUT GENERATION OF PATTERNED 3D CANCER CELL SPHEROIDS FROM ADHERENT MONOLAYERS AND CO-CULTURE WITH OTHER CELL TYPES

A micro-patterningĀ  method for spatially positioning tumor cells and controlling their morphology between a 3-D and a 2-D state is disclosed. The system consists of a microfluidic or robotic fluid delivery to first lay down a layer of the cell adhesive protein fibronectin, which serves as a mask for blocking tumor cell attachment as well as an adhesive substrate for other non-cancerou s cells. A second layer of a polycation such as PAH is then laid down over the fibronectin layer in a precisely defined pattern using either microfluidic or other means. Thishybrid surface can either be used as it is or further modified by treating with protein cross-l inking enzymes such as tissue transglutaminase or other reagents to alter cell behavior. Themethod/dev ice can either be used for testing anti-cancer drugs, for diagnostics, or for modeling the influence of neighboring cells on cancer cell phenotype and behaviors includ ing proliferation , migration, metastasis, and morphogen esis.
Inventors: 
Bhadriraju, Kiran

Reyes-Hernandez, Darwin R.

Internal Laboratory Ref #: 
17-002
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