Available Technology

A METHOD TO ENZYMATICALLY LABEL PROTEINS IN LIVING CELLS

Engineered sortase variants are shown to have promiscuous activity that allow proteinsto be tagged using a diverse array of small, commercially available amines, includingbioorthogonal functional groups. This technique can also be carried out in living microbial cells,enabling simple, inexpensive production of chemically functionalized proteins with no additionalpurification steps. The methods find use in the site specific conjugation of drugs, imaging probesand other chemical moieties to proteins and peptides.
Inventors: 
Glasgow, Jeff

Cochran, Jennifer

Salit, Marc L.

Patent Number: 
15/406,247
Internal Laboratory Ref #: 
16-011
Phone: 
301-975-2573
Email: 
tpo@nist.gov
Lab Representatives
Share to Facebook Share to Twitter Share to Google Plus Share to Linkedin