Available Technology

Device for Selectively Removing Cells from a Biological Sample

A variety of techniques have been used to isolate specific cells or cells populations from a histological sample under direct microscopic visualization. Techniques that involve manual or micro-manipulation devices to isolate individual cells based upon visible characteristics and/or immunological staining are labor intensive and can require an extended amount of time. The subject technology is a device and method of using the device to automatically identify cells reacting with light sensitive immunoreagent(s) (e.g. antibodies labeled with a light sensitive tag). The device may be used to selectively transfer the cells to a thermoplastic polymer film by exposing to a flashtube which selectively activates the immunoreagent. Activation of the immunoreagent bound to the cells causes the cells to selectively adhere to thermoplastic polymer film. Individual cells, populations of cells or specific regions of the tissue can be isolated using the device. Populations of cells can be sequentially isolated by employing immunoreagents that are activated by different wavelengths of light. The device can also be used to isolate cellular components (e.g. cell nuclei). The device can also be used for high throughput analysis of the samples.
Patent Abstract: 
A variety of techniques have been used to isolate specific cells or cells populations from a histological sample under direct microscopic visualization. Techniques that involve manual or micro-manipulation devices to isolate individual cells based upon visible characteristics and/or immunological staining are labor intensive and can require an extended amount of time. The subject technology is a device and method of using the device to automatically identify cells reacting with light sensitive immunoreagent(s) (e.g. antibodies labeled with a light sensitive tag). The device may be used to selectively transfer the cells to a thermoplastic polymer film by exposing to a flashtube which selectively activates the immunoreagent. Activation of the immunoreagent bound to the cells causes the cells to selectively adhere to thermoplastic polymer film. Individual cells, populations of cells or specific regions of the tissue can be isolated using the device. Populations of cells can be sequentially isolated by employing immunoreagents that are activated by different wavelengths of light. The device can also be used to isolate cellular components (e.g. cell nuclei). The device can also be used for high throughput analysis of the samples.
Benefits: 
Isolation of cells with specific characteristics can be achieved more quickly than those requiring manual microdissection. -The device can be used for automated high throughput analysis of histopathological samples.
applications: 
Inventors: 
Robert Bonner
Patent Number: 
62/016,571 PCT Application No. PCT/US2015/034930
Internal Laboratory Ref #: 
E-045-2014/0
Lab Representatives
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