Available Technology

Enhancing Absorption Measurement Sensitivity by Adjusting the Incident Light Spectrum

This invention is a spectroscopic method that adjusts the incident light intensity as a function of wavelength to flatten the solvent-only transmitted light spectrum over an interesting wavelength range. When a solvent has a strong absorption band within an interesting spectral region of analytes, a limited dynamic range of the detection system is dominated by the strong solvent absorption. This requires not only very thin optical pathlength but also very high analyte concentration. For example, due to the strong water absorption in the mid-IR range, aqueous protein samples should be prepared in a 6 or 8 um thick sample holder, and protein concentration should be as high as its solubility. This invention is intended to minimize the solvent contribution to the dynamic range of the detection system and to maximize the sensitivity of analyte-only absorbance, which can increase the available sample thickness and enhance the detection limit of analytes.

Patent Abstract: 

This invention is a spectroscopic method that adjusts the incident light intensity as a function of wavelength to flatten the solvent-only transmitted light spectrum over an interesting wavelength range. When a solvent has a strong absorption band within an interesting spectral region of analytes, a limited dynamic range of the detection system is dominated by the strong solvent absorption. This requires not only very thin optical pathlength but also very high analyte concentration. For example, due to the strong water absorption in the mid-IR range, aqueous protein samples should be prepared in a 6 or 8 um thick sample holder, and protein concentration should be as high as its solubility. This invention is intended to minimize the solvent contribution to the dynamic range of the detection system and to maximize the sensitivity of analyte-only absorbance, which can increase the available sample thickness and enhance the detection limit of analytes.

Inventors: 

Young Lee

Patent Number: 
Application 16/164859
Technology Type(s): 
Spectrometry
Internal Laboratory Ref #: 
18-007
Patent Status: 
pending 16/164859
Phone: 
301-975-2573
Email: 
tpo@nist.gov
Lab Representatives
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