Available Technology

VAC-BAC Shuttle Vector System for Generating Recombinant Poxviruses

This invention relates to a VAC-BAC shuttle vector system for the creation of recombinant poxviruses from DNA cloned in a bacterial artificial chromosome. A VAC-BAC is a bacterial artificial chromosome (BAC) containing a vaccinia virus genome (VAC) that can replicate in bacteria and produce infectious virus in mammalian cells.
Abstract: 
This invention relates to a VAC-BAC shuttle vector system for the creation of recombinant poxviruses from DNA cloned in a bacterial artificial chromosome. A VAC-BAC is a bacterial artificial chromosome (BAC) containing a vaccinia virus genome (VAC) that can replicate in bacteria and produce infectious virus in mammalian cells.
Benefits: 
VAC-BACs are clonally purified from bacterial colonies before virus reconstitution in mammalian cells. -Manipulation of DNA is much simpler and faster in bacteria than in mammalian cells. -Modified genomes can be characterized prior to virus reconstitution. -Only virus with modified genomes will be produced so that virus plaque isolations are not needed. -Generation of a stock of virus from a VAC-BAC is accomplished within a week rather than many weeks. -Multiple viruses can be generated at the same time since plaque purification is unnecessary.
applications: 
Inventors: 
Bernard Moss
Patent Number: 
PCT/US03/11183
Internal Laboratory Ref #: 
E-355-2001/2
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