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Laboratory Representative
Tech Transfer Website:
http://www.nhlbi.nih.gov/resources/tt/index.htmDescription
The National Heart, Lung, and Blood
Institute (NHLBI) provides leadership for a national program in
diseases of the heart, blood vessels, lung, and blood; blood
resources; and sleep disorders. Since October 1997, the NHLBI has
also had administrative responsibility for the NIH Woman's Health
Initiative. The Institute plans, conducts, fosters, and supports an
integrated and coordinated program of basic research, clinical
investigations and trials, observational studies, and demonstration
and education projects. Research is related to the causes,
prevention, diagnosis, and treatment of heart, blood vessel, lung,
and blood diseases; and sleep disorders. The NHLBI plans and
directs research in development and evaluation of interventions and
devices related to prevention, treatment, and rehabilitation of
patients suffering from such diseases and disorders. It also
supports research on clinical use of blood and all aspects of the
management of blood resources. Research is conducted in the
Institute's own laboratories and by scientific institutions and
individuals supported by research grants and contracts. For health
professionals and the public, the NHLBI conducts educational
activities, including development and dissemination of materials in
the above areas, with an emphasis on prevention. The NHLBI supports
research training and career development of new and established
researchers in fundamental sciences and clinical disciplines to
enable them to conduct basic and clinical research related to
heart, blood vessel, lung, and blood diseases; sleep disorders; and
blood resources through individual and institutional research
training awards and career development awards. The Institute
coordinates relevant activities in the above areas, including the
related causes of stroke, with other research institutes and
federal health programs. Relationships are maintained with
institutions and professional associations, and with international,
national, state, and local officials as well as voluntary agencies
and organizations working in the above areas.
Technology Disciplines
Displaying 1 - 10 of 14
Animal MRI Core
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The Animal Magnetic Resonance Imaging (MRI) Core develops and optimizes MRI methods for cardiovascular imaging of mice and rats. The Core provides imaging expertise, data analysis and interpretation, experimental design, and assistance in incorporating imaging studies into ACUC protocols. They can also incorporate additional imaging modalities through the MIF, such as computed tomography (CT), ultrasound and bioluminescence. The Core Staff facilitates implementation of imaging studies for investigators, performs imaging studies and teaches investigators and fellows. Studies are performed in the NIH Mouse Imaging Facility (MIF). Although the Core is primarily concerned with projects related to cardiovascular imaging in rodents, the team has also worked on more specialized applications, particularly in high resolution microimaging. Core research on techniques for microimaging embryos, particularly early stage embryos, was performed with the Lo group, Laboratory of Developmental Biology. Microimaging fibrosis in ex vivo heart tissue without fixation, and imaging localized contrast agent in this tissue without migration of the agent is another area of interest, with the Arai lab. Techniques for improved visualization of delayed contrast enhancement imaging in myocardial infarction in mice at 7T have been undertaken with the Boehm lab. Representative studies Cine cardiac imaging for ejection fraction, ventricle size and wall thicknesses High resolution imaging of myocardium for identification of infarct Imaging aorta and vessels in live mice and rats Imaging atherosclerotic plaque Perfusion of skeletal muscle Cellular imaging: magnetic labeling and tracking cell transplants Targeted MRI contrast agent research High resolution imaging of embryos High resolution imaging of fixed tissue
Animal Surgery and Resources Core
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Lab Reps:
James Hawkins
The ASR services for NHLBI research animals include: animal model development, surgery, surgical support, post-operative care as well as technical services such as radiography, blood & tissue collection, hematology, and blood chemistries.
Biochemistry Facility
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The Biochemistry Facility provides expert services and consultation in biochemical enzyme assays and protein purification. The facility currently features 1) Liquid chromatography - mass spectrometry (LC-MS) to detect the accurate mass of intact proteins, 2) High performance liquid chromatography (HPLC) equipped with radiochemical detectors and fluorescence detectors that allow for the purification of an enzyme or protein labeled with a radioisotope or fluorescence probe, and 3) Atomic absorption spectrometry to measure metal content in solution. All users can access equipment on a walk-in basis or may reserve time in advance. The reservation scheduler can be accessed through the 'iLab'. Instruments and services ESI-LC/MS, Agilent 6224 TOF and G1946D SQ Radiochemical detector, PerkinElmer Radiomatic 625TR w/ Agilent 1100 HPLC Fluorescence detector w/ Agilent 1100 HPLC Agilent 3100 OFFGel Fractionator Bio-Rad open column system in the cold room Anaerobic chamber Amino acid analysis using HPLC Metal content analysis using the graphite furnace Atomic absorption spectrometer
Bioinformatics and Systems Biology
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Lab Reps:
Xujing Wang
The Bioinformatics and Systems Biology (BISB) Core aims to assist investigators in overcoming the technical challenges in utilizing bioinformatics and systems biology techniques. The core will collaborate with principal investigators to incorporate systems biology approaches synergistically into their laboratory studies in order to speed the tempo of their research and develop transformative and translational results.
Biophysics Core
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The Biophysics Facility develops and optimizes methods to study macromolecular interactions, dynamics and stability. The Facility provides professional collaborations, consultation, training, and instrument access for NHLBI researchers. The Biophysics Facility currently has resources to study oligomeric state of biomolecular assemblies, perform measurements of affinity, stoichiometry, kinetics and thermodynamics of interactions between proteins, DNA and their cognate ligands. Biophysical characterization capabilities include measurements of molecular weight, shape, and conformation of biological macromolecules. Instruments Isothermal Titration Calorimetry (ITC) Differential Scanning Calorimetry (DSC) Surface Plasmon Resonance (SPR-Biacore) Analytical Ultracentrifugation Multi Angle Light Scattering (SEC-MALS/QELS) Circular Dichroism (CD) Fluorescence Stopped-flow
DNA Sequencing and Genomics Core
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Lab Reps:
Jun Zhu
The DNA Sequencing and Computational Biology Core (DSGC) was established in 2010 to meet the increasing demands of NHLBI investigators for next-generation sequencing. The main goal of the DSGC is to foster large-scale genomics research at DIR by providing access to cutting-edge sequencing instrumentation and genome technology. As an integral part of the research community at NHLBI, the DSGC provides standard and advanced services for experimental consultation, data acquisition and computational analysis.
Electron Microscopy Core
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The Electron Microscopy Core Facility provides advice, technical services, training and use of facilities to NHLBI intramural investigators who require electron microscopy (EM) to answer specific research questions. In general, this refers to questions involving subcellular, supramolecular or macromolecular structure at a level of resolution below that of the light microscope. The facility is equipped with two 120kV transmission electron microscopes and one scanning electron microscope as well as the preparative equipment for most biological EM applications. One transmission electron microscope is a current model equipped for electron tomography and automated montage acquisition. Equipment JEOL JEM 1400 Transmission Electron Microscope with AMT 11 Megapixel Bottom Mount Digital Camera and Serial EM software JEOL JEM 1200EXII transmission electron microscope equipped with an AMT XR-60 digital camera Hitachi S-3400N1 Scanning Electron Microscope with automated XY stage control Leica Ultracut UCT Ultramicrotome with EM FCS Cryo Accessory Leica Ultracut S Ultramicrotome RMC MTXL Ultramicrotome Tousimis Samdri-795 Critical Point Dryer EMS 575X Sputter Coater Denton Benchtop Turbo Vacuum Evaporator JEOL JFD-V Freeze Fracture/Freeze Etch Device Pelco Biowave Pro Microwave Processor Pelco EasiGlow Automated Plasma Cleaner
Flow Cytometry Core
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Lab Reps:
Philip Mccoy
The Flow Cytometry Core is a state-of-the-art facility to accommodate a range of cell sorting and analysis needs. A wide variety of applications are supported including high speed cell sorting, 15-color cell analysis, cytokine analysis by multiplex bead arrays, imaging flow cytometry, phoshoprotein analysis, and cell cycle analysis. Cytometers and Instruments 5 laser, 18 color FACSAria II 5 laser, 18 color FACSAria II 4 laser, 18 color LSR II 3 laser, 9 color CYAN 2 laser, 4 color FACSCalibur Amnis Imagestream Gen-X Mark II Luminex bead array analyzer Miltenyi MACS-Pro
iPSC Core
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Lab Reps:
Jizhong Zou
The induced Pluripotent Stem Cells (iPSC) Core was created in 2011 to accelerate stem cell research in the NHLBI by providing investigators consultation, technical services and training in human pluripotent stem cell technology. The major services that the Core currently provides are derivation, maintenance and characterization of human iPSCs. The Core also provides control iPSC lines and validated iPSC culture reagents. In addition to providing services and consultations to researchers from various disciplines, the Core staff is developing human iPSC gene-editing technologies, establishing lineage-specific differentiation protocols and collaborates with investigators in specific projects. Research conducted by the Core seeks to better understand the mechanisms underlying pluripotent stem cell self-renewal and differentiation to optimize culture conditions and operating procedures.
Light Microscopy Core
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Lab Reps:
Christian Combs
The Light Microscopy Core provides state-of-the-art equipment, training, and image processing capabilities to assist researchers in experiments involving light microscopy. Equipment within the facility includes several types of confocal microscopes, two multi-photon microscope, an FCS microscope, laser trap microscope, TIRF microscopes, several standard epi-fluorescence wide-field microscopes and specialty microscopes for super-resolution imaging and wide-area confocal imaging. This range of instruments allows for most types of modern imaging techniques from basic wide-field imaging to very high spatial and temporal resolution imaging for fixed and live-cell imaging to imaging organs and tissues in vivo. Image processing capabilities include image quantification, co-localization analysis, deconvolution, and 3D reconstruction as well as a custom in-house image processing programs for specific applications. The main points of contact in Bldg. 10 are Drs. Combs and Malide and Dr. Wu in Bldg. 50. Instruments Bldg. 10 Leica SP5 Multi-Photon (dual beam with OPO)/Confocal Microscope Zeiss LSM 510 META Confocal Microscope Zeiss LSM 510 UV Confocal Microscope Nikon Widefield Fluorescence Microscope Leica Fluorescence Stereomicroscope Modified Olympus IX81 Microscope (Structured Light, Ratiometric Calcium, Dual-beam TIRF Microscopy) Leica SP1 Confocal Microscope Leica TCS LSI Super-Zoom Confocal Olympus Fluoview 1000 Experimental Microcope Bldg. 50 Zeiss LSM 510 confocorr2 (FCS) Microscope Zeiss inverted Axiovert 100 Microscope with microinjector Zeiss LSM 510 META Confocal Microscope Zeiss 5 LIVE Confocal Microscope Zeiss LSM 780 Microscope Zeiss Stereomicroscope (SteREO Discovery V12) Zeiss up-right Axioplan Microscope Olympus IX 81 TIRF microscope Olympus XI81 with CSU-X1 spinning disk confocal and customer-made Laser Trap Zeiss LSM 710 2P Nikon STORM Super-resolutionMicroscope Coming soon: DeltaVison OMX Structured Light Microscope
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