Available Technology

Bacteriophage-Based Electrochemical Biosensor

A biosensor using bacteriophages in a sandwich-assay system for rapid detect of foodborne pathogens (i.e. Shiga-toxin producing Escherichia coli, STEC). The capture element includes a substrate and a bacteriophage and the detection element includes a bacteriophage and a signal amplification element. The target bacterium is sandwiched between the capture element and the detection element, and a quantifiable signal may be generated to measure the amount of bacteria in a sample. The biosensor uses direct sensing to detect the bacteria in the sample as opposed to indirect sensing methods.

Benefits
• Hand-held and portability for on-site rapid analysis and detection of significant groups of foodborne pathogens and toxins
• The sandwich assay uses direct sensing to detect the bacteria in the sample as opposed to indirect sensing methods
• Biosensors overcome the limitations of traditional foodborne pathogen detection such as tedious and time consuming by providing reliable, specific and highly sensitive platforms with shorter turnaround times
• Bacteriophages possess excellent host selectivity attributes

Applications
• To improve the screening and detection methods of foodborne pathogens in food and environmental samples, thus reducing foodborne illness, hospitalization and economic loss through safe foods

Patent Number: 
U.S. 10,768,177
Internal Laboratory Ref #: 
126.17
Lab Representatives
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