Available Technology

Mouse Macula Densa Cell Line

This technology provides a clonally derived macula densa cell line (MMDD1 cells) that closely mimics the known molecular expression pattern of native macula densa (MD) cells. MMDD1 cells are developed from SV-40 transgenic mice using fluorescence-activated cell sorting of renal tubular cells labeled with segment-specific fluorescent lectins. The MMDD1 cells of this technology express COX-2, bNOS, NKCC2, and ROMK, but not Tamm-Horsfall protein, and show rapid86Rb+uptake that is inhibited by a reduction in NaCl concentration and by bumetanide or furosemide. These MMDD1 cells provide a usefulin vitromodel for the study of Macula Densa function.
Patent Abstract: 
This technology provides a clonally derived macula densa cell line (MMDD1 cells) that closely mimics the known molecular expression pattern of native macula densa (MD) cells. MMDD1 cells are developed from SV-40 transgenic mice using fluorescence-activated cell sorting of renal tubular cells labeled with segment-specific fluorescent lectins. The MMDD1 cells of this technology express COX-2, bNOS, NKCC2, and ROMK, but not Tamm-Horsfall protein, and show rapid86Rb+uptake that is inhibited by a reduction in NaCl concentration and by bumetanide or furosemide. These MMDD1 cells provide a usefulin vitromodel for the study of Macula Densa function. Jurgen Schnermann (NIDDK)➽ more inventions... Research Tool -- patent protection is not being pursued for this technology T Yang, JM Park, L Arend, Y Huang, R Topaloglu, A Pasumarthy, H Praetorius, K Spring, JP Briggs, J Schnermann. Low chloride stimulation of prostaglandin E2release and cyclooxygenase-2 expression in a mouse macula densa cell line. J Biol Chem. 2000 Dec 1;275(48):37922-37929.PubMed: 10982805 The National Institute of Diabetes and Digestive and Kidney Diseases Kidney Disease Branch is seeking statements of capability or interest from parties interested in collaborative research to further develop, evaluate, or commercialize the clonally derived macula densa cell line (MMDD1 cells). Please contact Cindy Fuchs at 301-451-3636 for more information. Patrick McCue, Ph.D. Email:patrick.mccue@nih.gov Phone: 301-435-5560 E-234-2009/0 Updated:Jan 25, 2010
Internal Laboratory Ref #: 
E-234-2009/0
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