Available Technology

High Quality Tissue Formation Method

by using High-Density Spot Seeding (HDSS)

NASA Johnson Space Center seeks interested parties for the licensing and commercialization of the High Density Spot Seeding (HDSS) method patented technology used to create 2D and 3D tissue models. This method can be used to develop tissue models for a variety of applications including wound treatment, therapy, tissue modeling of skeletal muscle, cardiac muscle, nerve, and bone. The HDSS technique uses an easy four step method that does not require expensive reagents, such as specialized serum or growth factors and compared to traditional methods, HDSS has the potential to yield superior-quality tissue samples. This NASA Technology is available for your company to license and develop into a commercial product. NASA does not manufacture products for commercial sale.

Benefits

  • Simple: Uses natural process to generate a variety of tissues
  • Cost-Effective: Does not require use of expensive reagents
  • Superior Quality: Produces highly-aligned, linear, contracting tissue
  • Fast Production: Enables quick tissue generation with reliable timescale
  • Functional Cells: Produces contracting muscle tissue models

Applications

  • Basic Research and Development
  • Pharmaceutical Development
  • Regenerative Medicine
  • Drug Screening and Testing

The Technology

The HDSS technique can potentially facilitate tissue patching or wound repair in regenerative medicine field.
The HDSS technique can potentially facilitate tissue patching or wound repair in regenerative medicine field.

This technology is a simple, reproducible, and cost-effective process that creates 2D and 3D human tissue formations by high-density spot seeding (HDSS) of cells. The method entails the spot seeding of cells at a specific concentration onto a Petri dish, but without the need of extracellular matrix components. Cells are then incubated to allow attachment. The culture is rinsed with a medium to remove unattached cells and a growth medium is added to enable the cells at the periphery of the spot to proliferate and differentiate, outward from the center cells. This pattern of growth results in a 2D model of dense, organized, mature cells. It is proposed that the 2D formations can be stacked upon another via lamination process to create 3D tissues.
By forming tissue using this method, the technology enables the creation of unique models for R&D, pharmaceutical development and perhaps even regenerative medicine. For instance, for basic R&D, the study of mechanistic pathways involved in normal and/or diseased tissue becomes possible. This technology can also be used as an in-vitro tissue model for drug screening and toxicology testing in the pharmaceutical development field. The HDSS method may also be advantageous for high-throughput screening assays, where large volume of screenings are done simultaneously.

Download a PDF fact sheet for this technology.

Learn more: https://technology.nasa.gov/patent/MSC-TOPS-41 

 

Patent Number: 
8,735,1169,243,223
Internal Laboratory Ref #: 
MSC-TOPS-41 
Agency
NASA
State: 
Texas
Lab Representatives
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