Real-time PCR Assays for Selective Detection and Differentiation of B. pertussis, B. parapertussis and B. homesii

CDC researchers developed a real-time PCR assay targeting insertion sequence (IS481) and pertussis toxin subunit 1 (ptxS1) ofBordetella pertussis. This real-time nucleic acid assay offers rapid, sensitive, and quantitative results. The employed primers have been validated through extensive diagnostic testing of 41Bordetellaand 64 non-Bordetellaclinical isolates. This technology can be used to diagnose and distinguishB. pertussis,B. parapertussisandB. homesii, the three most commonBordetellahuman upper respiratory pathogens. A standalone assay or multi-faceted kit may be used.

Benefits

Validated for the three major pathogens responsible forBordetella-related upper respiratory infections -Rapid, sensitive and quantitative -Easily adapted to kit form -Useful as an added, internal control for presentBordetella pertussisdiagnostics

applications

Inventors:

Kathleen Tatti

Internal Laboratory Ref #:

E-193-2013/0

Centers for Disease Control and Prevention (CDC)

Agency

Dept. of Health and Human Services

Region

Southeast

State:

Georgia