Available Technology

Strains for the Production of Flavonoids from Glucose

Existing methods of producing flavonoids through fermentation require supplementation of the culture media with phenylpropanoic precursors -- expensive compounds that add significantly to production cost. Additionally, these methods also require two different culture media formulations to support protein expression and flavonoid production respectively. This two-stage culture introduces process complexity that complicates efforts to scale up flavonoid production. This invention addresses this problem with novel strains of microbes that enable flavonoid production in a single medium formulation without precursor supplementation.
This invention describes a set of Escherichia coli strains capable of producing naringenin -- an important flavonoid precursor -- using glucose as a source of nutrition. These E. coli cells were modified to express four yeast- and plant-derived genes to produce the enzymes making up the naringenin synthesis pathway. The genetic construct containing these genes was designed with a combination of inducible and constitutive promoters to balance their respective expression levels and maximize naringenin yield. Using these E. coli strains, the inventors have successfully achieved a final naringenin titer of 29 mg/L directly from glucose with no precursor supplementation.
Single minimal media formulation used throughout fermentation process - Eliminates need for precursor supplementation, thereby reducing substrate-related costs by several hundred fold
Gregory Stephanopoulos
Lab Representatives
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